Ukusetshenziswa Okuhlosiwe

I-Dengue NS1 Ag-IgG/IgM Combo Test ukuhlolwa kwe-chromatographic immunoassay okusheshayo kokutholwa kwekhwalithi yamasosha omzimba (i-IgG ne-IgM) kanye ne-antigen yegciwane lodenga i-NS1 kugciwane lodenga kuSerum noma ku-Plasma ukusiza ekuxilongweni kwegciwane leDengue.

[ISIFINYEZO]

I-Dengue fever yisifo esiyingozi esithathelwanayo esibangelwa igciwane lodenga elisakazwa omiyane.Ukutheleleka ngegciwane lodenga kungaholela ekuthelelekeni okuphindaphindekayo, udenga, imfiva yokopha udenga, imfiva yokopha udenga.Izimpawu zomtholampilo ezivamile zomkhuhlane wodenga zihlanganisa ukuqala kungazelelwe, umkhuhlane ophezulu, ikhanda elibuhlungu, izicubu ezibuhlungu, ubuhlungu bamathambo namalunga, ukuqubuka kwesikhumba, ukopha, ukwanda kwama-lymph node, ukwehla kwesibalo samangqamuzana egazi amhlophe, i-thrombocytopenia nokunye.Lesi sifo ngokuyisisekelo sisendaweni eshisayo kanye nedumela endaweni eshisayo, ngoba lesi sifo sisakazwa umiyane Aides, isizathu ukuthandwa has ezithile zonyaka, kube njalo ngonyaka ngokuvamile ngo May ~ November, umvuthwandaba in July ~ September.Endaweni entsha ewubhubhane, inani labantu livamise ukungenwa kalula yizifo, kodwa isigameko ikakhulukazi ngabantu abadala, endaweni ewubhubhane, isigameko ikakhulukazi izingane.

Isimiso

I-Dengue NS1 Ag-IgG/IgM Combo Test iyi-qualitative membrane strip based immunoassay ukuze kutholwe amasosha omzimba we-dengue virus (IgG ne-IgM) kanye ne-antigen ye-dengue virus NS1 kuSerum noma i-Plasma.

Ngokuhlolwa kwe-IgG/IgM :Idivayisi yokuhlola iqukethe: 1) i-burgundy colored conjugate pad equkethe ama-antigens emvilophu ahlanganiswe ne-Colloid gold (ama-dengue conjugates), 2) i-nitrocellulose membrane strip equkethe imigqa emibili yokuhlola (T1 kanye nemigqa ye-T2) kanye umugqa wokulawula (umugqa C).Ulayini we-T1 umbozwe ngaphambili ne-antibody ukuze kutholwe i-IgM anti-dengue, umugqa we-T2 umbozwe ngamasosha omzimba ukuze kutholwe i-IgG anti-dengue.Uma ivolumu eyanele yesampula yokuhlola ikhishelwa emthonjeni wesampula wekhasethi lokuhlola, isifanekiso sihamba ngesenzo se-capillary kulo lonke ikhasethi.I-anti-dengue ye-IgG, uma ikhona esibonelweni, izobophezela kuma-conjugates odenga.I-immunocomplex bese ibanjwa i-reagent eboshwe ngaphambili ebhendini ye-T2, yakhe umugqa we-T2 onombala o-burgundy, okhombisa umphumela wokuhlolwa oqondile we-dengue IgG futhi uphakamise ukutheleleka kwakamuva noma okuphindayo.I-anti-dengue ye-IgM uma ikhona kusifanekiso izobophezela kuma-conjugates odenga.I-immunocomplex bese ibanjwa i-reagent ehlanganiswe kulayini we-T1, yakhe umugqa we-T1 onemibala e-burgundy, ekhombisa umphumela wokuhlolwa oqondile we-dengue IgM futhi iphakamisa ukutheleleka okusha.Ukungabikho kwanoma yimiphi imigqa engu-T (T1 kanye ne-T2) kuphakamisa umphumela ongemuhle.

Ngokuhlolwa kwe-NS1: Kule nqubo yokuhlola, i-anti-Dengue NS1 antibody ayinyakazi esifundeni somugqa wokuhlola wekhasethi.Ngemuva kokuthi isampula Yegazi Eliphelele/iSerum/Plasma ibekwe esibonelweni kahle, sisabela ngezinhlayiya ezimboze i-anti-Dengue NS1 ezifakwe kuphedi wesifanekiso.Le ngxube ithutha ngokwechromatographic ngobude bomugqa wokuhlola futhi ihlanganyele ne-immubilised anti-Dengue NS1 antibody.Uma isifanekiso siqukethe i-antigen ye-dengue virus NS1, kuzovela umugqa onemibala endaweni yomugqa wokuhlola obonisa umphumela omuhle.Uma isifanekiso singenayo i-antigen ye-dengue virus NS1, umugqa onemibala ngeke uvele kulesi sifunda obonisa umphumela ongemuhle.

Ukuze usebenze njengesilawuli senqubo, ulayini onemibala uzohlala uvela endaweni yomugqa wokulawula obonisa ukuthi umthamo ofanele wesifanekiso sengeziwe futhi i-membrane wicking yenzekile.

Isitoreji Nokuzinza

Gcina njengoba kuhlanganiswe esikhwameni esivaliwe ekamelweni lokushisa noma esiqandisini (4-30℃ noma 40-86℉).Idivayisi yokuhlola izinzile ngedethi yokuphelelwa yisikhathi ephrintwe esikhwameni esivaliwe.

Ukuhlolwa kufanele kuhlale esikhwameni esivaliwe kuze kusetshenziswe.

Ukuqoqwa nokulungiselelwa kwesifanekiso

1. I-Dengue NS1 Ag-IgG/IgM Combo Test ingenziwa kuSerum noma kwi-Plasma.

2. Ukuqoqa igazi eliphelele, i-serum noma i-plasma specimens ngokulandela izinqubo zaselabhorethri zomtholampilo ezivamile.

3. Ukuhlolwa kufanele kwenziwe ngokushesha ngemva kokuqoqwa kwesifanekiso.Ungashiyi ama-specimens ekamelweni lokushisa isikhathi eside.Ukugcina isikhathi eside, ama-specimens kufanele agcinwe ngaphansi kuka-20℃.

4. Letha izibonelo ekamelweni lokushisa ngaphambi kokuhlolwa.Izifanekiso eziqandisiwe kufanele zincibilikiswe ngokuphelele futhi zixutshwe kahle ngaphambi kokuhlolwa.Izibonelo akufanele zifrizwe futhi zincibilikiswe ngokuphindaphindiwe.

Inqubo Yokuhlola

Vumela ukuhlola, isifanekiso, isigcinalwazi kanye/noma izilawuli ukuthi zifinyelele izinga lokushisa legumbi elingu-15-30℃ (59-86℉) ngaphambi kokuhlolwa.

1. Letha isikhwama ekamelweni lokushisa ngaphambi kokusivula.Susa idivayisi yokuhlola esikhwameni esivaliwe futhi uyisebenzise ngokushesha.Beka idivayisi yokuhlola endaweni ehlanzekile neleveli.

2. Ngokuhlolwa kwe-IgG/IgM :Bamba i-dropper ngokuqondile bese udlulisela iconsi elingu-1 lesifanekiso (cishe u-10μl) esibonelweni somthombo(S) wedivayisi yokuhlola, bese wengeza amaconsi angu-2 ebhafa (cishe u-70μl) bese uqala isibali sikhathi.Bheka umfanekiso ngezansi.

3. Ngokuhlolwa kwe-NS1: Bamba i-dropper ngokuqondile bese udlulisela amaconsi angu-8~10 e-serum noma i-plasma (cishe u-100μl) kumthombo wesampula(S) wedivayisi yokuhlola, bese uqala isibali sikhathi.Bheka umfanekiso ngezansi.

4. Linda kuze kuvele i(ama)layini anombala.Funda imiphumela ngemizuzu eyi-15.Ungahumushi umphumela ngemuva kwemizuzu engama-20.

Ukuhunyushwa Kwemiphumela

Okuhle:

Ngokuhlolwa kwe-IgG/IgM: Ulayini wokulawula kanye nokuvela okungenani umugqa owodwa wokuhlola kulwelwesi.Ukubukeka komugqa wokuhlola we-T2 kubonisa ukuba khona kwamasosha omzimba athile e-IgG yodenga.Ukubukeka komugqa wokuhlola we-T1 kubonisa ukuba khona kwamasosha omzimba athile e-IgM yodenga.Futhi uma ulayini we-T1 no-T2 uvela, kubonisa ukuthi ubukhona bamasosha omzimba akhethekile we-IgG ne-IgM.Uma ukuncipha kokugxilisa amasosha omzimba, umugqa wemiphumela uba buthaka.

Ngokuhlolwa kwe-NS1: Kuvela imigqa emibili.Umugqa owodwa kufanele uvele njalo endaweni yomugqa wokulawula(C), futhi omunye ulayini obonakalayo onombala kufanele uvele endaweni yomugqa wokuhlola.

Okubi:

Umugqa owodwa onombala uvela endaweni yokulawula(C).Awukho umugqa onombala ovela endaweni yomugqa wokuhlola.

Akuvumelekile: Ulayini wokulawula uyehluleka ukuvela.Ivolumu yesampula enganele noma izindlela zenqubo ezingalungile yizona zizathu okungenzeka kakhulu zokwehluleka komugqa wokulawula.Buyekeza inqubo bese uphinda ukuhlola ngedivayisi entsha yokuhlola.Uma inkinga iqhubeka, yeka ukusebenzisa ikhithi yokuhlola ngokushesha futhi uxhumane nomsabalalisi wangakini.