[UKUSEBENZISA OKUHLOSIWE]

I-Dengue NS1 Antigen Rapid Test Cassette/Strip iyi-lateral flow chromatographic immunoassay yokutholwa kwekhwalithi yama-antigen kumagciwane oDengue egazini lomuntu/iSerum/Plasma.Inikeza usizo ekuhlonzweni kokutheleleka ngegciwane lodenga.

[ISIFINYEZO]

I-Dengue fever yisifo esiyingozi esithathelwanayo esibangelwa igciwane lodenga elisakazwa omiyane.Ukutheleleka ngegciwane lodenga kungaholela ekuthelelekeni okuphindaphindekayo, udenga, imfiva yokopha udenga, imfiva yokopha udenga.Izimpawu zomtholampilo ezivamile zomkhuhlane wodenga zihlanganisa ukuqala kungazelelwe, umkhuhlane ophezulu, ikhanda elibuhlungu, izicubu ezibuhlungu, ubuhlungu bamathambo namalunga, ukuqubuka kwesikhumba, ukopha, ukwanda kwama-lymph node, ukwehla kwesibalo samangqamuzana egazi amhlophe, i-thrombocytopenia nokunye.Lesi sifo ngokuyisisekelo sisendaweni eshisayo kanye nedumela endaweni eshisayo, ngoba lesi sifo sisakazwa umiyane Aides, isizathu ukuthandwa has ezithile zonyaka, kube njalo ngonyaka ngokuvamile ngo May ~ November, umvuthwandaba in July ~ September.Endaweni entsha ewubhubhane, inani labantu livamise ukungenwa kalula yizifo, kodwa isigameko ikakhulukazi ngabantu abadala, endaweni ewubhubhane, isigameko ikakhulukazi izingane.

[ISIMISO]

I-Dengue NS1 Antigen Rapid Test Cassette/Strip iwukuhlolwa kokuzivikela komzimba okususelwe kumgomo wesu lesemishi le-antibody-sandwich.Ngesikhathi sokuhlolwa, i-anti-Dengue antibody ayinyakazi endaweni yomugqa wokuhlola wedivayisi.Ngemva kokuba isifanekiso Segazi Eliphelele/iSerum/Plasma sibekwe emthonjeni wesampula, sisabela nezinhlayiya ezimbozwe i-anti-Dengue antibody eziye zafakwa kuphedi wesifanekiso.Le ngxube ithutha ngokwechromatographic ngobude bomugqa wokuhlola futhi ihlanganyele ne-immubilised anti-Dengue antibody.Uma isifanekiso siqukethe i-antigen ye-dengue virus, kuzovela umugqa onemibala endaweni yomugqa wokuhlola obonisa umphumela omuhle.Uma isifanekiso singenayo i-antigen ye-dengue virus, umugqa onemibala ngeke uvele kulesi sifunda obonisa umphumela ongemuhle.Ukuze usebenze njengesilawuli senqubo, ulayini onemibala uzohlala uvela endaweni yomugqa wokulawula obonisa ukuthi umthamo ofanele wesifanekiso sengeziwe futhi i-membrane wicking yenzekile.

[ISITOREJI NOKUZInza]

Gcina njengoba kuhlanganiswe esikhwameni esivaliwe ezingeni lokushisa (4-30℃ noma 40-86℉).Ikhithi izinzile phakathi nedethi yokuphelelwa yisikhathi ephrintwe kulebula.

Uma usuvule isikhwama, ukuhlola kufanele kusetshenziswe phakathi nehora elilodwa.Ukuchayeka isikhathi eside endaweni eshisayo neswakeme kuzodala ukuwohloka komkhiqizo.

I-LOT kanye nosuku lokuphelelwa yisikhathi kwaphrintwa kulebula.

[UMBONO]

Ukuhlolwa kungasetshenziswa ukuhlola Igazi Eliphelele/iSerum/Plasma.

Qoqa isifanekiso segazi (esiqukethe i-EDTA, i-citrate noma i-heparin) ngokubhoboza imithambo ngokulandela izinqubo ezijwayelekile zaselabhorethri.

Hlukanisa i-serum noma i-plasma egazini ngokushesha ngangokunokwenzeka ukuze ugweme i-hemolytic.Sebenzisa kuphela izifanekiso ezingadiliziwe ezicacile.

Gcina izifanekiso ku-2-8℃ (36-46℉) uma zingahloliwe ngokushesha.Gcina ama-specimens ku-2-8℃ kufika ezinsukwini ezingu-7.Izibonelo kufanele ziqandiswe ku -20℃ (-4℉) ukuze kugcinwe isikhathi eside.Ungawaqandi amasampula egazi eliphelele.

Gwema imijikelezo eminingi yokuqandisa.Ngaphambi kokuhlola, letha izibonelo eziqandisiwe ekamelweni lokushisa kancane futhi uhlanganise kahle.Izibonelo eziqukethe i-particle ebonakalayo kufanele zicaciswe nge-centrifugation ngaphambi kokuhlolwa.

Ungasebenzisi amasampula abonisa i-gross lineman, i-gross hemolytic noma i-turbidity ukuze ugweme ukuphazamiseka ekuhumusheni imiphumela.

[INKQUBO YOKUHLOLA]

• Vumela idivayisi yokuhlola nezifanekiso ukuthi zilingane nezinga lokushisa (15-30℃noma 59-86℉) ngaphambi kokuhlolwa.
• [Okwe-Strip]

1. Khipha umugqa wokuhlola esikhwameni esivaliwe futhi uyisebenzise ngokushesha ngangokunokwenzeka.

2. Beka umugqa wokuhlola endaweni ehlanzekile nesezingeni.

3. Ngesampula ye-serum noma ye-plasma: Bamba i-dropper ngokuqondile bese udlulisela amaconsi angu-3 e-serum noma i-plasma (cishe u-100μl) kuphedi yesampula yomugqa wokuhlola, bese uqala isibali sikhathi.Bheka umfanekiso ngezansi.

4. ukuze uthole izibonelo zegazi eliphelele: Bamba i-dropper ngokuqondile bese udlulisela iconsi eli-1 legazi eliphelele (cishe u-35μl) kuphedi yesifanekiso yomugqa wokuhlola, bese wengeza amaconsi angu-2 ebhafa (cishe u-70μl) bese uqala isibali sikhathi.Bheka umfanekiso ngezansi.

5. Linda kuze kuvele i(ama)layini anombala.Funda imiphumela ngemizuzu eyi-15.Ungahumushi umphumela ngemuva kwemizuzu engama-20.