[UKUSEBENZISA OKUHLOSIWE]

I-Dengue IgG/IgM Rapid Test Cassette iyi-lateral flow chromatogram-raphic immunoassay yokutholwa kwekhwalithi yamasosha omzimba (i-IgG ne-IgM) kuya kugciwane lodenga egazini lomuntu lonke/iSerum/Plasma.Inikeza usizo ekuhlonzweni kokutheleleka ngegciwane lodenga.

[ISIFINYEZO]

I-Dengue fever yisifo esiyingozi esithathelwanayo esibangelwa igciwane lodenga elisakazwa omiyane.Ukutheleleka ngegciwane lodenga kungaholela ekuthelelekeni okuphindaphindekayo, udenga, imfiva yokopha udenga, imfiva yokopha udenga.Izimpawu zomtholampilo ezivamile zomkhuhlane wodenga zihlanganisa ukuqala kungazelelwe, umkhuhlane ophezulu, ikhanda elibuhlungu, izicubu ezibuhlungu, ubuhlungu bamathambo namalunga, ukuqubuka kwesikhumba, ukopha, ukwanda kwama-lymph node, ukwehla kwesibalo samangqamuzana egazi amhlophe, i-thrombocytopenia nokunye.Lesi sifo ngokuyisisekelo sisendaweni eshisayo kanye nedumela endaweni eshisayo, ngoba lesi sifo sisakazwa umiyane Aides, isizathu ukuthandwa has ezithile zonyaka, kube njalo ngonyaka ngokuvamile ngo May ~ November, umvuthwandaba in July ~ September.Endaweni entsha ewubhubhane, inani labantu livamise ukungenwa kalula yizifo, kodwa isigameko ikakhulukazi ngabantu abadala, endaweni ewubhubhane, isigameko ikakhulukazi izingane.

[ISIMISO]

I-Dengue IgG/IgM Rapid Test Cassette iyi-qualitative membrane strip based immunoassay ukuze kutholwe amasosha omzimba we-dengue virus (IgG ne-IgM) Egazini Lonke Lomuntu/iSerum/Plasma.Ikhasethi lokuhlola liqukethe: 1) i-burgundy colored conjugate pad equkethe ama-antigen emvilophu e-dengue ehlanganiswe negolide le-Colloid (ama-dengue conjugates), 2) i-nitrocellulose membrane strip equkethe imigqa yokuhlola emibili (imigqa ye-IgG ne-IgM) kanye nolayini wokulawula (umugqa C. ).Ulayini we-IgM uhlanganiswe kuqala ne-Mouse anti-Human IgM antibody, ulayini we-IgG uhlanganiswe ne-Mouse anti-Human IgG antibody.Uma ivolumu eyanele yesampula yokuhlola ikhishelwa emthonjeni wesampula wekhasethi lokuhlola, isifanekiso sihamba ngesenzo se-capillary kulo lonke ikhasethi.I-anti-dengue ye-IgM uma ikhona kusifanekiso izobophezela kuma-conjugates odenga.I-immunocomplex bese ibanjwa i-reagent ehlanganiswe kulayini we-IgM, yakhe umugqa we-IgM onemibala e-burgundy, ekhombisa umphumela wokuhlolwa oqondile we-dengue IgM futhi iphakamisa ukutheleleka okusha.I-anti-dengue ye-IgG, uma ikhona esibonelweni, izobophezela kuma-conjugates odenga.I-immunocomplex ibe isithathwa yi-reagent ehlanganiswe ngaphambili ebhendini ye-IgG, yakhe umugqa we-IgG onombala obomvana, okhombisa umphumela wokuhlolwa oqondile we-dengue IgG futhi iphakamisa ukutheleleka kwakamuva noma okuphindayo.Ukungabikho kwanoma yimiphi imigqa ye-T (IgG ne-IgM) kuphakamisa umphumela ongemuhle.Ukuze usebenze njengesilawuli senqubo, ulayini onemibala uzohlala uvela endaweni yomugqa wokulawula obonisa ukuthi umthamo ofanele wesifanekiso sengeziwe futhi i-membrane wicking yenzekile.

[ISITOREJI NOKUZInza]

Gcina njengoba kuhlanganiswe esikhwameni esivaliwe ezingeni lokushisa (4-30℃ noma 40-86℉).Ikhithi izinzile phakathi nedethi yokuphelelwa yisikhathi ephrintwe kulebula.

Uma usuvule isikhwama, ukuhlola kufanele kusetshenziswe phakathi nehora elilodwa.Ukuchayeka isikhathi eside endaweni eshisayo neswakeme kuzodala ukuwohloka komkhiqizo.

I-LOT kanye nosuku lokuphelelwa yisikhathi kwaphrintwa kulebula.

[UMBONO]

Ukuhlolwa kungasetshenziswa ukuhlola Igazi Eliphelele/iSerum/Plasma.

Ukuqoqa igazi eliphelele, i-serum noma izibonelo ze-plasma ngokulandela izinqubo zaselabhorethri zomtholampilo ezivamile.

Hlukanisa i-serum noma i-plasma egazini ngokushesha ngangokunokwenzeka ukuze ugweme i-hemolytic.Sebenzisa kuphela izifanekiso ezingadiliziwe ezicacile.

Gcina izifanekiso ku-2-8℃ (36-46℉) uma zingahloliwe ngokushesha.Gcina ama-specimens ku-2-8℃ kufika ezinsukwini ezingu-7.Izibonelo kufanele ziqandiswe ku-

-20℃ (-4℉) ukuze ugcine isikhathi eside.Ungawaqandi amasampula egazi eliphelele.

Gwema imijikelezo eminingi yokuqandisa.Ngaphambi kokuhlola, letha izibonelo eziqandisiwe ekamelweni lokushisa kancane futhi uhlanganise kahle.Izibonelo eziqukethe i-particle ebonakalayo kufanele zicaciswe nge-centrifugation ngaphambi kokuhlolwa.

Ungasebenzisi amasampula abonisa i-gross lineman, i-gross hemolytic noma i-turbidity ukuze ugweme ukuphazamiseka ekuhumusheni imiphumela.

[INKQUBO YOKUHLOLA]

Vumela idivayisi yokuhlola nezifanekiso ukuthi zilingane nezinga lokushisa (15-30℃ noma 59-86℉) ngaphambi kokuhlolwa.

1. Letha isikhwama ekamelweni lokushisa ngaphambi kokusivula.Susa ikhasethi lokuhlola esikhwameni esivaliwe futhi ulisebenzise ngokushesha ngangokunokwenzeka.

2. Beka ikhasethi lokuhlola endaweni ehlanzekile nesezingeni.

3. Bamba i-dropper ngokuqondile bese udlulisela iconsi elingu-1 lesifanekiso (cishe u-10μl) esibonelweni somthombo(S) wekhasethi lokuhlola, bese wengeza amaconsi angu-2 ebhafa (cishe u-70μl) bese uqala isibali sikhathi.Bheka umfanekiso ngezansi.

4. Linda kuze kuvele i(ama)layini anombala.Funda imiphumela ngemizuzu eyi-15.Ungahumushi umphumela ngemuva kwemizuzu engama-20.