Ikhasethi elisheshayo lokuhlola i-HIV antibody (Colloidal gold)
IKHITHI YOKUHLOLA OKUSHESHAYO I-HIV
ISIFINYEZO
Indlela evamile yokuthola ukutheleleka nge-HIV ukubuka ubukhona bamasosha omzimba egciwane ngendlela ye-EIA elandelwa ukuqinisekiswa nge-Western Blot.I-One Step HIV Test iwukuhlola okulula, okubonakalayo kwekhwalithi okuthola amasosha omzimba egazi Lonke Lomuntu/i-serum/plasma.Ukuhlolwa kusekelwe ku-immunochromatography futhi kunganikeza umphumela phakathi nemizuzu engu-15.
UKUSETSHENZISWA OKUHLOSIWE
Ukuhlolwa kwe-HIV kwe-One Step kuyi-Colloidal Gold ethuthukisiwe, i-Immunochromatoraphic Assay esheshayo yokutholwa kwekhwalithi yamasosha omzimba (i-HIV) ku-Human Whole Blood/Serum/Plasma.Lokhu kuhlolwa kuwukuhlola futhi wonke amaphozithivu kufanele aqinisekiswe kusetshenziswa okunye ukuhlola okufana ne-Western Blot.Ukuhlolwa kuhloselwe ukusetshenziswa Ochwepheshe Bezempilo kuphela.Kokubili ukuhlolwa kanye nemiphumela yokuhlolwa kuhloswe ukuthi kusetshenziswe ochwepheshe bezokwelapha nezomthetho kuphela, ngaphandle uma kugunyazwe ngenye indlela umthetho wezwe okusetshenziswa kulo.Ukuhlolwa akufanele kusetshenziswe ngaphandle kokugadwa okufanele.
ISIMISO SENKQUBO
Ukuhlolwa kuqala ngesampula elisetshenziswa emthonjeni wesampula kanye nokwengezwa kwe-diluent yesampula enikeziwe ngokushesha.I-HIV antigen-Colloidal Gold conjugate eshumekwe kuphedi yesampula ihlangana ne-antibody ye-HIV ekhona ku-serum noma ku-plasma, yakhe inkimbinkimbi ye-conjugate/HIV antibody.Njengoba ingxube ivunyelwa ukuthi ifuduke eduze komugqa wokuhlola, i-conjugate/antibody complex complex ithathwa iphrotheni ebopha amasosha omzimba A enganyakazi kulwelwesi olwakha ibhande elinemibala endaweni yokuhlola.Isampula elingenayo alikhiqizi umugqa wokuhlola ngenxa yokungabikho kwe-Colloidal Gold conjugate/antibody antibody complex.Ama-antigen asetshenziswa ekuhlolweni angamaphrotheni aphinde ahlanganiswe ahambisana nezindawo ezivikela kakhulu amasosha omzimba we-HIV.Ibhande lokulawula elinemibala endaweni yokulawula livela ekupheleni kwenqubo yokuhlola kungakhathaliseki umphumela wokuhlola.Le bhendi yokulawula ingumphumela we-Colloidal Gold conjugate ebophezela ku-anti-HIV antibody engakwazi ukuhamba kulwelwesi.Ulayini wokulawula ukhombisa ukuthi i-Colloidal Gold conjugate iyasebenza.Ukungabikho kwebhendi yokulawula kubonisa ukuthi ukuhlola akuvumelekile.
AMA-REAGENTS KANYE NEZINTO EZINIKEZELWAYO
Idivayisi yokuhlola i-foil ngayinye efakwe esikhwameni se-desiccant
• I-dropper yepulasitiki.
• I-Diluent eyisampula
• iphakheji Faka
IZINTO EZIDINGEKAYO KODWA AKUNIKEZWE
Izilawuli ezinhle nezingezinhle (zitholakala njengento ehlukile)
ISITOREJI NOKUZInza
Amakhithi okuhlola kufanele agcinwe ku-2-30℃ esikhwameni esivaliwe futhi ngaphansi kwezimo ezomile.
IZIXWAYISO NEZIQINISEKISO
1) Yonke imiphumela emihle kufanele iqinisekiswe ngenye indlela.
2) Phatha zonke izibonelo njengokungathi zingatheleleka.Gqoka amagilavu nezingubo zokuzivikela lapho uphatha izibonelo.
3) Amadivayisi asetshenziselwa ukuhlolwa kufanele afakwe ngokuzenzakalelayo ngaphambi kokulahlwa.
4) Ungasebenzisi izinto zekhithi ezingaphezu kwezinsuku zazo zokuphelelwa yisikhathi.
5) Ungashintshi ama-reagents kusuka kunkatho ehlukene.
UKUQOQWA KANYE NESIGCINISO ESAMPULA
1) Qoqa izibonelo Zegazi Eliphelele / ISerum / Plasma ngokulandela izinqubo zaselabhorethri zomtholampilo ezivamile.
2) Isitoreji: Igazi Eliphelele alikwazi ukuqandiswa.Isifanekiso kufanele sifakwe esiqandisini uma singasetshenziswa ngosuku olufanayo lokuqoqwa.Izifanekiso kufanele ziqandiswe uma zingasetshenziswa phakathi nezinsuku ezi-3 zokuqoqwa.Gwema ukuqhwaza nokuncibilikisa ama-specimens izikhathi ezingaphezu kwezi-2-3 ngaphambi kokuzisebenzisa.U-0.1% we-Sodium Azide ungengezwa kusifanekiso njengesivimbeli ngaphandle kokuthinta imiphumela yokuhlolwa.
INQUBO YOKUHLOLA
1) Usebenzisa i-dropper yepulasitiki evalekile yesampula, khipha iconsi elingu-1 (10μl) Legazi Eliphelele / iSerum / Plasma emthonjeni oyindilinga wekhadi lokuhlola.
2) Engeza amaconsi ama-2 e-Sample Diluent emthonjeni wesampula, ngokushesha ngemva kokuba isifanekiso sengezwe, kusukela ku-vial ethiphu ye-dropper diluent vial (noma konke okuqukethwe ku-ampule yokuhlola eyodwa).
3) Humusha imiphumela yokuhlolwa ngemizuzu eyi-15.
Amanothi:
1) Ukusebenzisa inani elanele lesampula le-diluent kubalulekile ukuze uthole umphumela wokuhlola ovumelekile.Uma ukufuduka (ukumanziswa kolwelwesi) kungabonwa efasiteleni lokuhlola ngemva komzuzu owodwa, engeza enye iconsi elilodwa le-diluent emthonjeni wesampula.
2) Imiphumela emihle ingavela ngokushesha nje ngomzuzu owodwa ngesampula enamazinga aphezulu amasosha omzimba e-HIV.
3) Ungatoliki imiphumela ngemuva kwemizuzu engama-20
UKUFUNDA IMIPHUMELA YOKUHLOLA
1)Okuhle: Kokubili ibhendi yokuhlola ebomvu e-purplish kanye nebhande lokulawula elibomvu elibomvu kuvela kulwelwesi.Ukuncipha kwe-antibody, ibhande lokuhlola liba ntekenteke.
2) Okubi: Ibhande lokulawula elibomvu kuphela elivela kulwelwesi.Ukungabikho kwebhendi yokuhlola kubonisa umphumela ongemuhle.
3)Umphumela ongavumelekile:Kufanele kuhlale kukhona ibhande lokulawula elibomvu elibomvu endaweni yokulawula, kungakhathaliseki umphumela wokuhlolwa.Uma ibhendi yokulawula ingabonwa, ukuhlolwa kuthathwa njengokungavumelekile.Phinda ukuhlola usebenzisa idivayisi entsha yokuhlola.
Qaphela: Kuyinto evamile ukuba nebhande lokulawula elikhanyiswe kancane elinamasampuli aqine kakhulu, inqobo nje uma libonakala ngokusobala.
UMKHAWULO
1) Kuphela okucacile, okusha, nokugeleza kwamahhala kwe-Whole Blood/Serum/Plasma kuphela okungasetshenziswa kulokhu kuhlolwa.
2) Amasampula amasha angcono kakhulu kodwa amasampula aqandisiwe angasetshenziswa.Uma isampula lifriziwe, kufanele livunyelwe ukuthi lincibilike lime mpo futhi lihlolwe ukuthi alimanzi yini.Igazi Eliphelele alikwazi ukumiswa.
3) Ungawunyakazisi isampula.Faka i-pipette ngezansi nje kobuso besampula ukuze uqoqe I-Specimen.
