(FMDV-O) Ikhithi Yokuhlola Igciwane Lesifo Sonyawo Nomlomo
[Ingemuva]
Umkhiqizo unganquma ngokushesha isimo samasosha omzimba ohlobo lwe-O yegciwane lesifo sonyawo nomlomo ezilwaneni ngokuhlolwa kwe-immunochromatographic kumasampula e-serum noma e-plasma kanye nokutholwa kwegciwane lesifo somlomo nohlobo lwe-O amasosha omzimba athile, ukuze kunikezwe inkomba yomtholampilo. ukuhlonzwa kwegciwane lesifo sonyawo nomlomo uhlobo O.
[Isimiso sokutholwa]
Lo mkhiqizo usebenzisa i-immunochromatography esheshayo ukuze kutholwe amasosha omzimba ohlobo lwegciwane lesifo somlomo no-O.Ngemuva kokuthi leyo sampula yengezwe emgodini wesampula we-adde yekhadi lokutholwa, uma uhlobo lwegciwane lesifo sonyawo nomlomo lukhona kusampula, i-antibody ingahlanganisa ngokuqondile negciwane lesifo sonyawo nomlomo uhlobo lwe-antigen ephawuliwe. ngegolide eliyi-colloidal ukuze kwakheke inkomba ehamba eduze kolwelwesi lwe-chromatographic futhi ithwetshulwa iphrotheni embozwe ngaphambili kulwelwesi lwe-chromatographic ukuze kwakhe umugqa wokutholwa obomvu wewayini endaweni t yegobolondo le-clam.Uma ingekho i-antibody yohlobo lwe-FMDV kusampula, awukho umugqa obonakalayo owakhiwe endaweni ye-T.Ngaphezu kwalokho, ulayini we-C nawo waklanywa kule sistimu ukuze kuqinisekiswe ukusebenza kahle kokuhlolwa.Ulayini uzofakwa umbala wokunegethivu noma ube phozithivu, ngaphandle kwalokho kuzothiwa awuvumelekile.
[Ukwakheka komkhiqizo]
Ikhithi yokuhlola i-antibody ye-Foot-and-mouth (izikhwama ezingama-50/ ibhokisi)
I-dropper (i-1 pc/isikhwama)
I-Desiccant (1pc/isikhwama)
Umyalelo (1 pc/ibhokisi)
[Ukusetshenziswa]
Sicela ufunde imiyalelo yokusebenza ngokucophelela ngaphambi kokuhlolwa, bese ubuyisela ikhadi lokuhlola kanye nesampula ukuze ihlolwe ekamelweni lokushisa elingu-15–25℃.
1. Igazi elisha laqoqwa, i-serum yahlukaniswa ngokuma, noma amasampula e-plasma atholwe nge-centrifugation, futhi amasampula aqinisekiswa ukuthi awaguquguquki noma anezimvula.
2. Khipha ucezu lwephakethe lekhadi lokuhlola bese ulidabula, khipha ikhadi lokuhlola, lenze lilingane endaweni yokusebenza.
3. Faka isampula kahle "S", engeza amaconsi angu-2-3 (cishe 70-100 mL) wesampula.
4. Okubonwayo phakathi kwemizuzu emi-5-10, okungavumelekile ngemva kwemizuzu eyi-15.
[Isinqumo somphumela]
* Okuhle (+): Amabhande abomvu ewayini omugqa wokulawula C kanye nolayini wokuthola u-T ubonise ukuthi isampula iqukethe i-anti-and-mouth antibody yohlobo A.
* Inegethivu (-): Awukho umbala owenziwe ku-T-ray yokuhlola, okubonisa ukuthi isampula lalingenayo i-antibody yohlobo A lwe-foot-and-mouth.
* Okungavumelekile: Awukho Ulayini C we-QC noma Ibhodi Elimhlophe elikhona elibonisa inqubo engalungile noma ikhadi elingavumelekile.Sicela uphinde uhlole.
[Izinyathelo zokuzivikela]
1. Sicela usebenzise ikhadi lokuhlola phakathi nesikhathi sesiqinisekiso futhi phakathi nehora elilodwa ngemva kokuvula:
2. Lapho uhlola ukugwema ukukhanya kwelanga kanye nokuphephetha kwefeni kagesi;
3. Zama ukuthi ungathinti indawo yefilimu emhlophe maphakathi nekhadi lokutholwa;
4. I-dropper yesampula ayikwazi ukuxutshwa, ukuze ugweme ukungcoliswa okuphambene;
5. Ungasebenzisi isampula lokuhlanjululwa kwemvelo elingahlinzekwanga nalesi sici;
6. Ngemuva kokusetshenziswa kwekhadi lokubona kufanele kuthathwe njengokucutshungulwa kwezimpahla eziyingozi;
[Imikhawulo yohlelo lokusebenza]
Lo mkhiqizo uyikhithi yokuxilonga i-immunological futhi isetshenziselwa ukuhlinzeka ngemiphumela yokuhlolwa kwekhwalithi yokutholwa kwezifo ezifuywayo.Uma kukhona ukungabaza mayelana nemiphumela yokuhlolwa, sicela usebenzise ezinye izindlela zokuxilonga (ezifana ne-PCR, ukuhlolwa kwe-pathogen isolation, njll.) ukuze wenze ukuhlaziya okwengeziwe nokuxilonga amasampula atholiwe.Xhumana nodokotela wezilwane wangakini ukuze uthole ukuxilongwa kwe-pathological.
[Isitoreji nokuphelelwa yisikhathi]
Lo mkhiqizo kufanele ugcinwe ku-2 ℃–40℃ endaweni epholile, eyomile ekude nokukhanya futhi hhayi iqhwa;Isebenza izinyanga ezingama-24.Bona iphakheji yangaphandle yedethi yokuphelelwa yisikhathi kanye nenombolo yeqoqo.
