(TP ) Ikhasethi le-Syphilis Blood Rapid Test
Ikhasethi Yekhithi Yokuhlola Igazi le-TP Syphilis
ISIFINYEZO
Indlela evamile yokuthola ukutheleleka nge-TP isetshenziselwa ukutholwa kwekhwalithi ye-in vitro ye-Syphilis (TP) Amasosha omzimba egazini lomuntu lonke, i-serum noma amasampula e-plasma.Indlela yegolide ye-Colloidalfuthi anganikeza umphumela phakathi nemizuzu eyi-15.
UKUSETSHENZISWA OKUHLOSIWE
I-One Step TP Test iyigolide le-Colloidal elithuthukisiwe,.Ngokomtholampilo, lo mkhiqizo usetshenziselwa ikakhulukazi ukuxilongwa kwe-Treponema pallidum ukutheleleka.Lo mkhiqizo usetshenziswa abasebenzi bezokwelapha kuphela.
ISIMISO SENKQUBO
Umkhiqizo wamukela isimiso se-double antigen sandwich.Uma isampula iqukethe i-Treponema pallidum antibody, i-Treponema pallidum antibody kusampula isabela ne-colloidal yegolide elibhalwe ukuthi-syphilis recombinant antigen 1 kuphedi yokubopha ukuze yakhe inkimbinkimbi enelebula ye-antigen-antibody.Inkimbinkimbi i-chromato-graphed phambili ngesenzo se-capillary futhi ithathwa yi-recombinant syphilis antigen 2 embozwe endaweni ye-detecticn(umugqa we-T) wolwelwesi lwe-nitrocellulose, bese kuvela ibhande elibomvu.I-complex iyaqhubeka nokuba i-chromatographed ibheke phezulu, futhi umaka wegolide we-chicken lgY colloidal uthathwa yi-anti-chicken lgY antibody ehlanganiswe endaweni yokulawula ikhwalithi (umugqa C) we-nitrocellulose membrane, bese kuvela ibhande elibomvu.Uma okuqukethwe kwe-analyte kusampula kungaphansi komkhawulo wokuthola ubuncane, indawo yokuthola (umugqa we-T) awuthuthukisi umbala.
ISAKHIWO ESIPHAMBILI
1.Iphedi yokuhlola, ngayinye ihlanganiswe esikhwameni se-aluminium foil(1piece/isikhwama,1/5/10/25/50ucezu/ikhithi
2. Utshani bepulasitiki obulahlwayo(1picce/isikhwama,1/5/10/25/50 ucezu/ikhithi)
3.Isikhwama sikadoti wezokwelapha(1picce/isikhwama,1/5/10/25/50 iziqephu/ikhithi)
4.Imanuwali yokufundisa(1ikhophi/isikhwama,ikhophi eyi-1/ikhithi)
Qaphela: Izingxenye ezikumakhithi ezinombolo zeqoqo ezahlukene azishintsheki.
IZINTO ONGAZITHANDELA
I-diluent yesampula (ucezu olu-1/isikhwama,1/5/10/25/50 ucezu/ikhithi)
Iphedi likakotini lotshwala(ucezu/isikhwama,1/5/10/25/50 iziqephu/ikhithi)
口 Inalithi yokuqoqwa kwegazi(ucezu/isikhwama esi-1,1/5/10/25/50 ucezu/ikhithi)
IZINTO EZIDINGEKAYO KODWA AKUNIKEZWE
Izilawuli ezinhle nezingezinhle (zitholakala njengento ehlukile)
ISITOREJI NOKUZInza
Ukupakishwa kwangempela kufanele kugcinwe endaweni eyomile ku-4-30 ℃ evikelwe ekukhanyeni, futhi ungafrizi.
IZIXWAYISO NEZIQINISEKISO
Ikhithi izinzile phakathi nedethi yokuphelelwa yisikhathi ephrintwe kulebula.Iphedi yokuhlola kufanele isetshenziswe lingakapheli ihora ngaphansi kwesimo esingu-4-30℃nomswakama<65% ngemva kokuba isikhwama se-aluminium foil singapakishiwe.Kunconywa ukuyisebenzisa ngokushesha ngaphansi kokushisa okuphezulu noma izimo zomswakama ophezulu.
UKUQOQWA KANYE NESIGCINISO ESAMPULA
2. Isampula yokugcina
2.1 Igazi lonke;Amashubhu e-Anticoagulant asetshenziselwa ukuqoqwa kwegazi, futhi ajwayelekileama-anticoagulants angasetshenziswa;uma amasampula egazi eliphelele engakwazi ukusetshenziswa ngokushesha ngemva kwalokhoeqoqweni, angagcinwa ku-2-8°C izinsuku ezi-3, futhi amasampula awakwazi ukuqandiswa.
2.2 I-Serum/plasma: Isampula ingagcinwa ku-2-8℃ izinsuku eziyi-7, futhi kufaneleigcinwe ku -20 ℃ ukuze igcinwe isikhathi eside.
3.Kumele kusetshenziswe amasampula angewona awe-hemolyzed kuphela.Amasampula ane-hemolyzed kakhulu kufanelekwenziwe isampula kabusha.
4 Amasampula afakwe esiqandisini kufanele abuyiselwe ezingeni lokushisa elilingana negumbi ngaphambi kokuhlolwa.Iamasampula aqandisiwe kufanele ancibilikiswe ngokuphelele, afudunyezwe futhi axutshwe ngokulinganayo ngaphambilisebenzisa.Ungafrizi futhi uncibilike ngokuphindaphindiwe
INQUBO YOKUHLOLA
1) Usebenzisa i-dropper yepulasitiki evalekile yesampula, khipha iconsi elingu-1 (10μl) Legazi Eliphelele / iSerum / Plasma emthonjeni oyindilinga wekhadi lokuhlola.
2) Engeza amaconsi ama-2 e-Sample Diluent emthonjeni wesampula, ngokushesha ngemva kokuba isifanekiso sengezwe, kusukela ku-vial ethiphu ye-dropper diluent vial (noma konke okuqukethwe ku-ampule yokuhlola eyodwa).
3) Humusha imiphumela yokuhlolwa ngemizuzu eyi-15.
Amanothi:
1) Ukusebenzisa inani elanele lesampula le-diluent kubalulekile ukuze uthole umphumela wokuhlola ovumelekile.Uma ukufuduka (ukumanziswa kolwelwesi) kungabonwa efasiteleni lokuhlola ngemva komzuzu owodwa, engeza enye iconsi elilodwa le-diluent emthonjeni wesampula.
2) Imiphumela emihle ingavela ngokushesha nje ngomzuzu owodwa wesampula enamazinga aphezulu amasosha omzimba e-TP.
3) Ungatoliki imiphumela ngemuva kwemizuzu engama-20
UKUFUNDA IMIPHUMELA YOKUHLOLA
1)Okuhle: Kokubili ibhendi yokuhlola ebomvu e-purplish kanye nebhande lokulawula elibomvu elibomvu kuvela kulwelwesi.Ukuncipha kwe-antibody, ibhande lokuhlola liba ntekenteke.
2) Okubi: Ibhande lokulawula elibomvu kuphela elivela kulwelwesi.Ukungabikho kwebhendi yokuhlola kubonisa umphumela ongemuhle.
3)Umphumela ongavumelekile:Kufanele kuhlale kukhona ibhande lokulawula elibomvu elibomvu endaweni yokulawula, kungakhathaliseki umphumela wokuhlolwa.Uma ibhendi yokulawula ingabonwa, ukuhlolwa kuthathwa njengokungavumelekile.Phinda ukuhlola usebenzisa idivayisi entsha yokuhlola.
Qaphela: Kuyinto evamile ukuba nebhande lokulawula elikhanyiswe kancane elinamasampuli aqine kakhulu, inqobo nje uma libonakala ngokusobala.
UMKHAWULO
1) Kuphela okucacile, okusha, nokugeleza kwamahhala kwe-Whole Blood/Serum/Plasma kuphela okungasetshenziswa kulokhu kuhlolwa.
2) Amasampula amasha angcono kakhulu kodwa amasampula aqandisiwe angasetshenziswa.Uma isampula lifriziwe, kufanele livunyelwe ukuthi lincibilike lime mpo futhi lihlolwe ukuthi alimanzi yini.Igazi Eliphelele alikwazi ukumiswa.
3) Ungawunyakazisi isampula.Faka i-pipette ngezansi nje kobuso besampula ukuze uqoqe I-Specimen.
