Ikhasethi lokuhlola okusheshayo kwenja yokukhulelwa ngaphambi kwesikhathi(igazi/i-serum/plasma)
Igama lomkhiqizo
Ikhithi Yokuhlola Ukukhulelwa Kwenja Ngaphambi Kokuqala
Uhlobo lwesampula: I-Serum, i-plasma, igazi eliphelele
Izinga lokushisa lesitoreji
2°C - 30°C
Izithako Nokuqukethwe
Ikhadi lokutholwa kwenja isencane (amakhophi ayi-10/ibhokisi)
I-Centrifuge tube (amakhophi ayi-10/ibhokisi)
I-Eyedropper (1/isikhwama)
I-Desiccant (isikhwama esi-1/isikhwama)
I-Diluent (amabhodlela ayi-10/ibhokisi, 1.0mL/ ibhodlela)
Umyalelo (1 ikhophi/ibhokisi)
[Ukusetshenziswa okuhlosiwe]
Umkhiqizo ungathola ngokushesha i-relaxin ku-serum yenja ne-plasma ngokuhlolwa kwe-immunochromatographic kumasampuli egazi lezinja.
[Usiminyaka yobudala]
Funda i-IFU ngokuphelele ngaphambi kokuhlola, vumela idivayisi yokuhlola nezifanekiso zilingane nezinga lokushisa legumbi(15～25℃) ngaphambi kokuhlolwa.
Indlela :
1. Igazi elisha lonke laqoqwa, i-serum yatholwa ngokuma, noma amasampula e-plasma atholwe nge-centrifugation, futhi amasampuli aqinisekiswa ukuthi awaguquguquki noma anezimvula.
2. Khipha ucezu lwephakethe lekhadi lokuhlola bese ulidabula, khipha ikhadi lokuhlola, lenze lilingane endaweni yokusebenza.
3. Kusampula kahle "S", kancane kancane engeza amaconsi angu-3-4 we-serum noma isampula ye-plasma usebenzisa i-pipette.
4. Ukubhekwa kwenziwa cishe ngemva kwemizuzu eyi-10, kodwa imiphumela yayingavumelekile ngemva kwemizuzu engu-15.
Amanothi: Lo mkhiqizo uklanyelwe ukuthi uhlolwe ezinsukwini eziyi-15 ngemva kokukhwelana (cishe u-3.8ng/mL) futhi kungakapheli izinsuku eziyi-15 ngemva kokukhwelana.Isikhathi esifanelekile sokuhlolwa yizinsuku ezingama-22-28 ngemuva kokukhwelana.Ngenxa yomehluko ngamunye, ukunegethivu okungamanga ngenxa yokuhlolwa kokuqala kuthatha iviki elingu-1 ukuqeda.
[Isinqumo somphumela]
* Okuhle (+): Amabhande abomvu ewayini omugqa wokulawula C kanye nolayini wokuthola u-T ubonise ukuthi isampula iqukethe i-anti-and-mouth antibody yohlobo A.
* Inegethivu (-): Awukho umbala owenziwe ku-T-ray yokuhlola, okubonisa ukuthi isampula lalingenayo i-antibody yohlobo A lwe-foot-and-mouth.
* Okungavumelekile: Awukho Ulayini C we-QC noma Ibhodi Elimhlophe elikhona elibonisa inqubo engalungile noma ikhadi elingavumelekile.Sicela uphinde uhlole.
[Izinyathelo zokuzivikela]
1. Sicela usebenzise ikhadi lokuhlola phakathi nesikhathi sesiqinisekiso futhi phakathi nehora elilodwa ngemva kokuvula:
2. Lapho uhlola ukugwema ukukhanya kwelanga kanye nokuphephetha kwefeni kagesi;
3. Zama ukuthi ungathinti indawo yefilimu emhlophe maphakathi nekhadi lokutholwa;
4. I-dropper yesampula ayikwazi ukuxutshwa, ukuze ugweme ukungcoliswa okuphambene;
5. Ungasebenzisi isampula lokuhlanjululwa kwemvelo elingahlinzekwanga nalesi sici;
6. Ngemuva kokusetshenziswa kwekhadi lokubona kufanele kuthathwe njengokucutshungulwa kwezimpahla eziyingozi;
[Imikhawulo yohlelo lokusebenza]
Lo mkhiqizo uyikhithi yokuxilonga i-immunological futhi isetshenziselwa ukuhlinzeka ngemiphumela yokuhlolwa kwekhwalithi yokutholwa kwezifo ezifuywayo.Uma kukhona ukungabaza mayelana nemiphumela yokuhlolwa, sicela usebenzise ezinye izindlela zokuxilonga (ezifana ne-PCR, ukuhlolwa kwe-pathogen isolation, njll.) ukuze wenze ukuhlaziya okwengeziwe nokuxilonga amasampula atholiwe.Xhumana nodokotela wezilwane wangakini ukuze uthole ukuxilongwa kwe-pathological.
[Isitoreji nokuphelelwa yisikhathi]
Lo mkhiqizo kufanele ugcinwe ku-2℃–40℃ endaweni epholile, eyomile ekude nokukhanya futhi hhayi iqhwa;Isebenza izinyanga ezingama-24.
Bona iphakheji yangaphandle yedethi yokuphelelwa yisikhathi kanye nenombolo yeqoqo.
