(CSF) Ikhithi yokuhlola ye-elisa ye-swine fever yakudala
Igama lomkhiqizo
Ikhithi Yokuhlola I-Classical Swine Fever Antibody
Uhlobo lwesampula: I-Serum, i-plasma
Izinga lokushisa lesitoreji
2°C - 30°C
Izithako Nokuqukethwe
Ikhithi yokuhlola i-antibody fever yakudala (izikhwama ezingama-50/ ibhokisi)
I-dropper (1 pc/isikhwama)
I-Desiccant (i-1 pc/isikhwama)
I-Diluent (amabhodlela angu-50/ibhokisi, 1.0mL/ ibhodlela)
Umyalelo (1 pc/ibhokisi)
[Ukusetshenziswa okuhlosiwe]
Okusunguliwe kuveza ikhadi le-antibody legciwane lemfiva le-swine fever elitholakala ngokushesha, okuyi-transverse flow immunochromatographic assay yokutholwa kwekhwalithi ye-classical swine fever virus antibody (CSFV Ab) ku-pig serum noma amasampula e-plasma.
[Usiminyaka yobudala]
Funda i-IFU ngokuphelele ngaphambi kokuhlola, vumela idivayisi yokuhlola nezifanekiso zilingane nezinga lokushisa legumbi(15～25℃) ngaphambi kokuhlolwa.
Indlela :
1. Qoqa amasampula e-serum entsha noma e-plasma futhi uqinisekise ukuthi amasampula awanayo i-turbidity noma intlenga.
2. Khipha ucezu lwephakethe lekhadi lokuhlola bese ulidabula, khipha ikhadi lokuhlola, lenze lilingane endaweni yokusebenza.
3. Yengeza kancane kancane amaconsi angu-2-3 (cishe ama-80-100uL) e-serum noma i-plasma ku-Sample Well "S" usebenzisa i-pipette.
4. Ukubuka phakathi kwemizuzu engu-5-10, okungavumelekile ngemva kwemizuzu engu-15.
[Isinqumo somphumela]
* Okuhle (+): Amabhande abomvu ewayini omugqa wokulawula C kanye nolayini wokuthola u-T ubonise ukuthi isampula iqukethe i-anti-and-mouth antibody yohlobo A.
* Inegethivu (-): Awukho umbala owenziwe ku-T-ray yokuhlola, okubonisa ukuthi isampula lalingenayo i-antibody yohlobo A lwe-foot-and-mouth.
* Okungavumelekile: Awukho Ulayini C we-QC noma Ibhodi Elimhlophe elikhona elibonisa inqubo engalungile noma ikhadi elingavumelekile.Sicela uphinde uhlole.
[Izinyathelo zokuzivikela]
1. Sicela usebenzise ikhadi lokuhlola phakathi nesikhathi sesiqinisekiso futhi phakathi nehora elilodwa ngemva kokuvula:
2. Lapho uhlola ukugwema ukukhanya kwelanga kanye nokuphephetha kwefeni kagesi;
3. Zama ukuthi ungathinti indawo yefilimu emhlophe maphakathi nekhadi lokutholwa;
4. I-dropper yesampula ayikwazi ukuxutshwa, ukuze ugweme ukungcoliswa okuphambene;
5. Ungasebenzisi isampula lokuhlanjululwa kwemvelo elingahlinzekwanga nalesi sici;
6. Ngemuva kokusetshenziswa kwekhadi lokubona kufanele kuthathwe njengokucutshungulwa kwezimpahla eziyingozi;
[Imikhawulo yohlelo lokusebenza]
Lo mkhiqizo uyikhithi yokuxilonga i-immunological futhi isetshenziselwa ukuhlinzeka ngemiphumela yokuhlolwa kwekhwalithi yokutholwa kwezifo ezifuywayo.Uma kukhona ukungabaza mayelana nemiphumela yokuhlolwa, sicela usebenzise ezinye izindlela zokuxilonga (ezifana ne-PCR, ukuhlolwa kwe-pathogen isolation, njll.) ukuze wenze ukuhlaziya okwengeziwe nokuxilonga amasampula atholiwe.Xhumana nodokotela wezilwane wangakini ukuze uthole ukuxilongwa kwe-pathological.
[Isitoreji nokuphelelwa yisikhathi]
Lo mkhiqizo kufanele ugcinwe ku-2℃–40℃ endaweni epholile, eyomile ekude nokukhanya futhi hhayi iqhwa;Isebenza izinyanga ezingama-24.
Bona iphakheji yangaphandle yedethi yokuphelelwa yisikhathi kanye nenombolo yeqoqo.
