Ukuhlolwa kweCanine Heartworm Antigen Test CHW Ag Test
Igama lomkhiqizo
Ikhasethi le-CHW Antigen Rapid Test (Igazi Eliphelele/iSerum/Plasma)
Isikhathi sokutholwa: 5-10 imizuzu
Amasampula okuhlola: Igazi Eliphelele/iSerum/Plasma
Izinga lokushisa lesitoreji
2°C - 30°C
[AMA-REAGENT KANYE NEZIMALI]
Ikhasethi Lokuhlola I-CHW Ag (amakhophi/ibhokisi)
I-dropper (1/isikhwama)
I-Desiccant (isikhwama esi-1/isikhwama)
I-Diluent (1 ibhodlela/ibhokisi)
Umyalelo (ikhophi/ibhokisi eli-1)
I-CHW Antigen Rapid Test Cassette (Igazi Eliphelele/iSerum/Plasma) iyisemishi i-lateral flow immunochromatographic assay yokutholwa kwekhwalithi ye-canine heartworm antigen (CHW Ag) egazini le-canine lonke noma i-serum ne-plasma.
[izinyathelo zokusebenza]
- Vumela zonke izinto ezisetshenziswayo, okuhlanganisa isifanekiso nedivayisi yokuhlola, zibuyele ku-15-25℃ ngaphambi kokwenza isilingo.
- Khipha ikhadi lokuhlola esikhwameni se-foil bese ulibeka livundlile.
- Qoqa u-20μL wesampula esilungisiwe (i-serum, i-plasma noma igazi eliphelele) ebhodleleni le-assay buffer futhi uhlanganise kahle.Bese uphonsa amathonsi ama-3 (okungaba ngu-120μL) wesampula ehlanjululwe embobeni yesampula "S" yekhadi lokuhlola.
- Humusha umphumela ngemizuzu emi-5-10.Umphumela ngemuva kwemizuzu eyi-10 uthathwa njengongavumelekile.
[Isinqumo somphumela]
-Okuhle (+): Ukuba khona kwakho kokubili komugqa “C” kanye nomugqa wendawo “T”, kungakhathaliseki ukuthi umugqa ongu-T ucacile noma awucacile.
-Negative (-): Kuvela umugqa C ocacile kuphela.Awukho umugqa we-T.
1. Sicela usebenzise ikhadi lokuhlola phakathi nesikhathi sesiqinisekiso futhi phakathi nehora elilodwa ngemva kokuvula:
2. Lapho kuhlolwa ukugwema ukukhanya kwelanga kanye nokuphephetha kwefeni kagesi;
3. Zama ukuthi ungathinti indawo yefilimu emhlophe maphakathi nekhadi lokutholwa;
4. I-dropper yesampula ayikwazi ukuxutshwa, ukuze ugweme ukungcoliswa okuphambene;
5. Ungasebenzisi isampula lokuhlanjululwa kwemvelo elingahlinzekwanga nalesi sici;
6. Ngemuva kokusetshenziswa kwekhadi lokubona kufanele kuthathwe njengokucutshungulwa kwezimpahla eziyingozi;
[Imikhawulo yohlelo lokusebenza]
Lo mkhiqizo uyikhithi yokuxilonga i-immunological futhi isetshenziselwa ukuhlinzeka ngemiphumela yokuhlolwa kwekhwalithi yokutholwa kwezifo ezifuywayo.Uma kukhona ukungabaza mayelana nemiphumela yokuhlolwa, sicela usebenzise ezinye izindlela zokuxilonga (ezifana ne-PCR, ukuhlolwa kwe-pathogen isolation, njll.) ukuze wenze ukuhlaziya okwengeziwe nokuxilonga amasampula atholiwe.Xhumana nodokotela wezilwane wangakini ukuze uthole ukuxilongwa kwe-pathological.
[Isitoreji nokuphelelwa yisikhathi]
Lo mkhiqizo kufanele ugcinwe ku-2℃–40℃ endaweni epholile, eyomile ekude nokukhanya futhi hhayi iqhwa;Isebenza izinyanga ezingama-24.
Bona iphakheji yangaphandle yedethi yokuphelelwa yisikhathi kanye nenombolo yeqoqo.
