Ikhithi Yokuhlola Esheshayo yase-African Swine Fever Antigen ((igolide eliyi-Colloidal)
Igama lomkhiqizo
Ukuhlolwa kwe-antigen virus ye-African Swine fever (Colloidal gold)
Isibonelo: I-Serum
Ukusebenza kabusha: Ingulube
Izinga lokushisa lesitoreji
2°C - 30°C
Izithako Nokuqukethwe
Ukuhlolwa kwe-antigen virus ye-African Swine fever (Colloidal gold) ukuhlolwa okungu-25/ibhokisi
Isampula sebhafa 1 ibhodlela/ibhokisi (1 OmL/ibhodlela)
I-Dropper engu-25 ama-pcs / ibhokisi
Incwadi Yeziqondiso 1 ukuphakela/ibhokisi
[Ukusetshenziswa okuhlosiwe]
Ilungele ukutholwa ngokushesha kwegciwane le-African swine fever ku-porcine serum
[Usiminyaka yobudala]
Funda i-IFU ngokuphelele ngaphambi kokuhlola, vumela idivayisi yokuhlola nezifanekiso zilingane nezinga lokushisa legumbi(15～30 ℃ noma 59-86℉) ngaphambi kokuhlolwa.
Indlela : Nge-serum
(1)Khipha isivivinyoikhasethiukusukaivaliweisikhwama bese uyisebenzisa phakathi nehora elilodwa ngemva kwalokhokuvulwe.
(2) Place umkhiqizo phezu kwedeski flat.
(3) Thatha i-1 mL yesampula yegazi lengulube eqoqiwe uyifake ku-1.5 mL centrifuge tube, i-centrifuge ku-3500r/min imizuzu emi-5, thatha isampula ye-serum ephezuluii-dropper, engeza i-drop engu-1 emgodini wesampula.
(4) Add 2 amathonsikwe-bufferemgodini wesampula wokuhlolwaikhasethi, futhi uqale ukubeka isikhathi.
[Isinqumo somphumela]
* Okuhle (+): Amabhande abomvu ewayini omugqa wokulawula C kanye nolayini wokuthola u-T ubonise ukuthi isampula iqukethe i-anti-and-mouth antibody yohlobo A.
* Inegethivu (-): Awukho umbala owenziwe ku-T-ray yokuhlola, okubonisa ukuthi isampula lalingenayo i-antibody yohlobo A lwe-foot-and-mouth.
* Okungavumelekile: Awukho Ulayini C we-QC noma Ibhodi Elimhlophe elikhona elibonisa inqubo engalungile noma ikhadi elingavumelekile.Sicela uphinde uhlole.
[Izinyathelo zokuzivikela]
1. Sicela usebenzise ikhadi lokuhlola phakathi nesikhathi sesiqinisekiso futhi phakathi nehora elilodwa ngemva kokuvula:
2. Lapho uhlola ukugwema ukukhanya kwelanga kanye nokuphephetha kwefeni kagesi;
3. Zama ukuthi ungathinti indawo yefilimu emhlophe maphakathi nekhadi lokutholwa;
4. I-dropper yesampula ayikwazi ukuxutshwa, ukuze ugweme ukungcoliswa okuphambene;
5. Ungasebenzisi isampula lokuhlanjululwa kwemvelo elingahlinzekwanga nalesi sici;
6. Ngemuva kokusetshenziswa kwekhadi lokubona kufanele kuthathwe njengokucutshungulwa kwezimpahla eziyingozi;
[Imikhawulo yohlelo lokusebenza]
Lo mkhiqizo uyikhithi yokuxilonga i-immunological futhi isetshenziselwa ukuhlinzeka ngemiphumela yokuhlolwa kwekhwalithi yokutholwa kwezifo ezifuywayo.Uma kukhona ukungabaza mayelana nemiphumela yokuhlolwa, sicela usebenzise ezinye izindlela zokuxilonga (ezifana ne-PCR, ukuhlolwa kwe-pathogen isolation, njll.) ukuze wenze ukuhlaziya okwengeziwe nokuxilonga amasampula atholiwe.Xhumana nodokotela wezilwane wangakini ukuze uthole ukuxilongwa kwe-pathological.
