[UKUSEBENZISA OKUHLOSIWE]

I-Influenza A+B Rapid Test iyi-assay esheshayo ebonakalayo yokuhlolwa kwekhwalithi, okucatshangelwayo kwe-antigens yegciwane lomkhuhlane A no-B enza ama-swabs omphimbo kanye nezibonelo ze-nasopharyngeal swab.Ukuhlolwa kuhloswe ukuthi kusetshenziswe njengosizo ekuxilongeni okusheshayo okuhlukile kohlobo lomkhuhlane oyingozi A kanye nohlobo B lokutheleleka ngegciwane.

ISIMISO

Ikhasethi Lokuhlola Okusheshayo Lomkhuhlane A+B lithola ama-antigen egciwane lomkhuhlane A no-B ngokutolikwa okubonakalayo kokuthuthukiswa kombala emgqeni.Amasosha omzimba e-anti-influenza A no-B awanyakazi endaweni yokuhlola A no-B yolwelwesi ngokulandelana.Ngesikhathi sokuhlolwa, isifanekiso esikhishiwe sibhekana nama-anti-influenza A no-B amasosha omzimba ahlanganiswe ezinhlayiyeni ezinemibala futhi ahlanganiswe ngaphambili kuphedi yesampula yokuhlolwa.Ingxube ibe isihamba nge-membrane ngesenzo se-capillary futhi ixhumana nama-reagents kulwelwesi.Uma kukhona ama-antigens egciwane lomkhuhlane A no-B owanele kusifanekiso, amabhendi anombala azokwakheka endaweni yokuhlola yolwelwesi.Ukuba khona kwebhande elinemibala esifundeni A kanye/noma B kubonisa umphumela omuhle kuma-antigen athile egciwane, kuyilapho ukungabikho kwawo kubonisa umphumela omubi.Ukubonakala kwebhande elinemibala endaweni yokulawula kusebenza njengokulawula kwenqubo, okubonisa ukuthi umthamo ofanele we-specimen wengeziwe futhi i-membrane wicking yenzekile.

ISITOREJI NOKUZInza

1.Ikhithi kufanele igcinwe ku-2-30°C kuze kube usuku lokuphelelwa yisikhathi luphrintiwe esikhwameni esivaliwe.

2.Ukuhlolwa kufanele kuhlale esikhwameni esivaliwe kuze kusetshenziswe.

3.Ungaqandi.

4.Ukunakekelwa kufanele kuthathwe ukuze kuvikelwe izingxenye zekhithi ekungcoleni.Ungasebenzisi uma kunobufakazi bokungcoliswa kwamagciwane noma imvula.Ukungcoliswa kwebhayoloji kwezinto ezisetshenziswayo zokusaphaza, iziqukathi noma ama-reagents kungaholela emiphumeleni engamanga.

INQUBO

Letha izivivinyo, izibonelo, kanye/noma izilawuli ekamelweni lokushisa (15-30°C) ngaphambi kokusetshenziswa.

1.Khipha isivivinyo esikhwameni saso esivaliwe, bese usibeka endaweni ehlanzekile, esezingeni.Lebula iKhasethi ngesiguli noma umazisi wokulawula.Ukuze uthole imiphumela engcono kakhulu, ukuhlolwa kufanele kwenziwe lingakapheli ihora elilodwa.

2. Hlanganisa ngobumnene isisombululo se-reagent ye-Extraction.Engeza amaconsi angu-6 Esixazululo Sokukhipha kushubhu Lokukhipha.

3.Beka isampula ye-swab yesiguli ku-Extraction Tube.Roll swab okungenani izikhathi ezingu-10 ngenkathi ucindezela i-swab phansi kanye nohlangothi lwe-Extraction Tube.Gingqa ikhanda le-swab phakathi kwe-Extraction Tube njengoba ulisusa.Zama ukukhulula uketshezi oluningi ngangokunokwenzeka.Lahla i-swab esetshenzisiwe ngokuhambisana nephrothokholi yakho yokulahla imfucuza ye-biohazard.

4.Faka ithiphu leshubhu, bese wengeza amaconsi angu-4 esampula ekhishiwe emthonjeni wesampula.Ungayibambi noma uhambise Ikhasethi Lokuhlola kuze kuqedwe ukuhlola futhi kulungele ukufundwa.

5.Njengoba ukuhlola kuqala ukusebenza, umbala uzohamba unqamule ulwelwesi.Linda ukuthi amabhendi anombala avele.Umphumela kufanele ufundwe ngemizuzu eyi-10.Ungahumushi umphumela ngemuva kwemizuzu engama-20.

UKUTONGULWA KWEMIPHUMELA

Vumela ikhasethi yokuhlola nezifanekiso zilingane nezinga lokushisa (15-30℃ noma 59-86℉) ngaphambi kokuhlolwa.

1. Khipha ikhasethi lokuhlola esikhwameni esivaliwe.

2. Hlehlisa ishubhu lokukhipha isifanekiso, Ukubamba isizinda somfanekiso

ithubhu imile, dlulisa amaconsi ama-3 (cishe i-100μl) esibonelweni

kahle(Izi-) zekhasethi lokuhlola, bese uqala isibali sikhathi.Bheka umfanekiso ngezansi.

Linda imigqa enemibala ukuthi ivele.Humusha imiphumela yokuhlolwa emizuzwini eyi-15.Ungafundi imiphumela ngemuva kwemizuzu engama-20.

IMIKHAWULO YOKUHLOLA

1.Ikhasethi Lokuhlola Okusheshayo Lomkhuhlane A+B elokusetshenziswa kochwepheshe be-in vitro ukuxilonga, futhi kufanele lisetshenziselwe kuphela ukutholwa kwekhwalithi yomkhuhlane A kanye/noma u-B.

2.I-etiology yokutheleleka kokuphefumula okubangelwa ama-microorganisms ngaphandle kwegciwane lomkhuhlane A noma u-B ngeke isungulwe ngalokhu kuhlolwa.Ikhasethi Lokuhlola Okusheshayo Lomkhuhlane A+B liyakwazi ukubona kokubili izinhlayiya zomkhuhlane osebenzayo nezingenzeki.Ukusebenza kwekhasethi Lokuhlola Okusheshayo Lomkhuhlane A+B kuncike ekulayisheni kwe-antigen futhi kungase kungahambelani nesiko leseli elenziwe kumfanekiso ofanayo.

3.Uma umphumela wokuhlolwa ungalungile futhi izimpawu zomtholampilo ziqhubeka, ukuhlolwa okwengeziwe kusetshenziswa ezinye izindlela zomtholampilo kuyanconywa.Umphumela ongemuhle awusho nganoma yisiphi isikhathi ukuba khona kwama-antigens egciwane lomkhuhlane A kanye/noma B kusifanekiso, njengoba angase abe khona ngaphansi kwezinga eliphansi lokutholwa kokuhlolwa.Njengazo zonke izivivinyo zokuxilonga, ukuxilongwa okuqinisekisiwe kufanele kwenziwe ngudokotela kuphela ngemva kokuba konke okutholwe emitholampilo naselabhorethri sekuhlolwe.

4.Ukufaneleka kwekhasethi Lokuhlola Okusheshayo Lomkhuhlane A+B akufakazelwanga ukuze kukhonjwe noma kuqinisekiswe ukuhlukaniswa kwe-cell culture.

5.Ukuqoqwa kwesifanekiso esinganele noma esingafanele, ukugcinwa, kanye nokuthutha kungase kuveze umphumela wokuhlolwa onegethivu okungamanga.

6.Nakuba lokhu kuhlola kubonisiwe kuthola amagciwane omkhuhlane wezinyoni akhulisiwe, okuhlanganisa igciwane le-avian influenza A subtype H5N1, izici zokusebenza zalokhu kuhlolwa nezifanekiso ezivela kubantu abangenwe i-H5N1 noma amanye amagciwane omkhuhlane wezinyoni akwaziwa.

7.Izimpawu zokusebenza zomkhuhlane A zasungulwa lapho umkhuhlane A/H3 kanye ne-A/H1 kungamagciwane omkhuhlane A ahamba phambili.Uma kuvela amanye amagciwane omkhuhlane A, izici zokusebenza zingahluka.

8.Izingane zivame ukulahla igciwane isikhathi eside kunabantu abadala, okungase kubangele umehluko ekuzweleni phakathi kwabantu abadala kanye nezingane.

9.Amanani abikezelayo amahle kanye namabi ancike kakhulu ekusakazekeni.Imiphumela yokuhlolwa engelona iqiniso ingenzeka kakhulu ngezikhathi zomkhuhlane omncane lapho ukusabalala kumaphakathi kuya phansi.

QAPHELA:

1.Ukushuba kombala endaweni yokuhlola (A/B) kungase kuhluke kuye ngokugxila kokuhlaziya okukhona kusifanekiso.Ngakho-ke, noma imuphi umthunzi wombala endaweni yokuhlola (A/B) kufanele kubhekwe njengento enhle.Sicela uqaphele ukuthi lokhu ukuhlolwa kwekhwalithi kuphela, futhi akukwazi ukunquma ukugcwala kwabahlaziyi kusifanekiso.

2.Ivolumu yesampula enganele, inqubo yokusebenza engalungile noma izivivinyo eziphelelwe yisikhathi yizizathu okungenzeka kakhulu zokuhluleka kwebhande lokulawula.