I-COVID-19 Antigen Rapid Test Cassette ( Amathe )
I-COVID-19 Neutralizing Antibody Rapid Test Cassette (Colloidal Gold) iwukuhlola okusheshayo kwe-chromatographic ukuze kutholwe ikhwalithi yamasosha omzimba angathathi hlangothi ku-COVID-19 egazini lonke lomuntu, i-serum, noma i-plasma njengosizo ekuhlonzeni ukuba khona kwamasosha omzimba angathathi hlangothi. ku-COVID-19.
Ama-coronavirus anoveli awohlobo lwe-β.I-COVID-19 yisifo esithathelwanayo sokuphefumula esinamandla.Ngokuvamile abantu bayathinteka.Njengamanje, iziguli ezingenwe yi-coronavirus yenoveli ziwumthombo oyinhloko wokutheleleka;abantu abane-asymptomatic abangenwe yileli gciwane bangaba umthombo othathelwanayo.Ngokusekelwe ophenyweni lwamanje lwe-epidemiological, isikhathi sokufukamela siyizinsuku ezi-1 kuya kweziyi-14, izikhathi eziningi izinsuku ezi-3 kuya kweziyi-7.Izimpawu eziyinhloko zihlanganisa umkhuhlane, ukukhathala nokukhwehlela okomile.Ukucinana kwamakhala, ikhala eligijimayo, umphimbo obuhlungu, i-myalgia kanye nesifo sohudo kutholakala ezimweni ezimbalwa.
I-COVID-19 Neutralizing Antibody Rapid Test Cassette (Igolide Le-Colloidal) isivivinyo esisheshayo esisebenzisa inhlanganisela yezinhlayiya ezinombala we-S-RBD antigen ukuze kutholwe amasosha omzimba angathathi hlangothi ku-COVID-19 egazini lomuntu lonke, i-serum, noma i-plasma.
I-COVID-19 Neutralizing Antibody Rapid Test Cassette (i-Colloidal Gold) iwulwelwesi olusekelwe ku-immunoassay ukuze kutholwe amasosha omzimba angathathi hlangothi ku-COVID-19 egazini lonke, i-serum noma i-plasma.Ulwelwesi lumbozwe ngaphambili nge-Angiotensin I Eguqulayo I-Enzyme 2 (ACE2) endaweni yomugqa wokuhlola womucu.Ngesikhathi sokuhlolwa, lonke igazi, i-serum noma isampula ye-plasma isabela ngegolide elihlanganisiwe le-colloid le-S-RBD.Ingxube ithuthela phezulu kulwelwesi lwe-chromatographical ngesenzo se-capillary ukuze iphendule nge-ACE2 kulwelwesi futhi ikhiqize umugqa onemibala.Ukuba khona kwalo mugqa onemibala kubonisa umphumela omubi, kuyilapho ukungabi khona kwawo kubonisa umphumela omuhle.Ukuze usebenze njengesilawuli senqubo, ulayini onemibala uzohlala ushintsha usuka Okuluhlaza Kabuhlaza Kwesibhakabhaka uye Obomvu endaweni yokulawula umugqa, okubonisa ukuthi umthamo ofanele we-specimen wengeziwe futhi i-membrane wicking yenzekile.
| Amadivayisi okuhlola apakishwe ngawodwana | Idivayisi ngayinye iqukethe umugqa onama-conjugate anemibala nama-reagents asebenzayo asakazwe ngaphambilini ezindaweni ezihambisanayo. |
| Amapayipi alahlwayo | Ukuze ungeze ama-specimens sebenzisa |
| Ibhafa | I-phosphate buffered saline kanye ne-preservative |
| Faka iphakheji | Ukuze uthole imiyalelo yokusebenza |
Izinto Ezinikeziwe
| ● Hlola amadivayisi | ●Amaconsi |
| ●Ibhafa | ● Faka iphakheji |
Izinto Ezidingekayo Kodwa Azinikezwa
| ●Iziqukathi zokuqoqa izifanekiso | ●Isibali sikhathi |
| ●I-Centrifuge |
1. Okokusetshenziswa kochwepheshe be-in vitro diagnostic kuphela.
2. Ungasebenzisi ngemva kosuku lokuphelelwa yisikhathi olukhonjiswe ephaketheni.Ungasebenzisi ukuhlolwa uma isikhwama se-foil sonakalisiwe.Ungaphinde usebenzise ukuhlola.
3. Isixazululo se-reagent yokukhipha siqukethe isixazululo sikasawoti uma isixazululo sithinta isikhumba noma iso, sishaywa ngamanzi amaningi.
4. Gwema ukungcoliswa ngokwehlukana kwezibonelo ngokusebenzisa isiqukathi esisha sokuqoqa isifanekiso sesampula ngayinye etholiwe.
5. Funda yonke inqubo ngokucophelela ngaphambi kokuhlolwa.
6. Ungadli, ungaphuzi noma ungabhemi endaweni lapho kuphathwa khona ama-specimens namakhithi.Phatha zonke izifanekiso njengokungathi ziqukethe izinto ezithathelwanayo.Qaphela izinyathelo ezimisiwe zokubhekana nobungozi be-microbiological kuyo yonke inqubo futhi ulandele izinqubo ezijwayelekile zokulahlwa okufanele kwezibonelo.Gqoka izingubo zokuzivikela njengamajazi aselabhorethri, amagilavu alahlwayo kanye nokuvikela amehlo lapho izibonelo zihlolwa.
7. Uma ukutheleleka nge-coronavirus yenoveli kusolwa ngokusekelwe kumbandela wokuhlolwa wamanje womtholampilo kanye ne-epidemiological otuswe iziphathimandla zezempilo yomphakathi, ama-specimens kufanele aqoqwe ngezinyathelo ezifanele zokulawula ukutheleleka kumanoveli ama-coronavirus futhi athunyelwe emnyangweni wezempilo wesifunda noma wendawo ukuze ayohlolwa.Isiko legciwane akufanele lizanywe kulezi zimo ngaphandle uma i-BSL 3+ itholakala ukuze yamukelwe nezifanekiso zesiko.
8. Ungashintshisani noma uhlanganise ama-reagents avela ezindaweni ezahlukene.
9. Umswakama nezinga lokushisa kungaba nomthelela omubi emiphumeleni.
10. Izinto zokuhlola ezisetshenzisiwe kufanele zilahlwe ngokuhambisana nemithetho yendawo.
1. Ikhithi kufanele igcinwe ku-2-30 ° C kuze kube usuku lokuphelelwa yisikhathi luphrintiwe esikhwameni esivaliwe.
2. Ukuhlolwa kufanele kuhlale esikhwameni esivaliwe kuze kusetshenziswe.
3. Ungaqandi.
4. Kufanele kuqashelwe ukuvikela izingxenye zekhithi ekungcoleni.Ungasebenzisi uma kunobufakazi bokungcoliswa kwamagciwane noma imvula.Ukungcoliswa kwebhayoloji kwezinto ezisetshenziswayo zokusaphaza, iziqukathi noma ama-reagents kungaholela emiphumeleni engamanga.
Cabangela noma yiziphi izinto zemvelaphi yomuntu njengezithathelwanayo futhi uziphathe usebenzisa izinqubo ezijwayelekile ze-biosafety.
I-Capillary Whole Blood
Geza isandla sesiguli bese usivumela ukuba some.Hlanza isandla ngaphandle kokuthinta ukubhoboza.Gcoba isikhumba nge-lancet oyinyumba.Sula uphawu lokuqala lwegazi.Hlikihla kancane isandla kusukela esihlakaleni kuya entendeni yesandla ukuya emunweni ukuze wenze iconsi legazi eliyindilinga phezu kwendawo yokubhoboza.Engeza isampula ye-Fingerstick Whole Blood kudivayisi yokuhlola ngokusebenzisa ishubhu le-capillary noma amaconsi alengayo.
I-venous Whole Blood:
Qoqa isifanekiso segazi eshubhu le-lavender, eluhlaza okwesibhakabhaka noma eluhlaza okotshani (eliqukethe i-EDTA, i-citrate noma i-heparin, ngokulandelanayo ku-Vacutainer®) ngokubhoboza imithambo.
I-Plasma
Qoqa isifanekiso segazi eshubhu le-lavender, eluhlaza okwesibhakabhaka noma eluhlaza okotshani (eliqukethe i-EDTA, i-citrate noma i-heparin, ngokulandelanayo ku-Vacutainer®) ngokubhoboza imithambo.Hlukanisa i-plasma nge-centrifugation.Khipha ngokucophelela i-plasma eshubhuni elisha elilebulwe ngaphambili.
I-Serum
Qoqa isifanekiso segazi eshubhuni leqoqo eliphezulu elibomvu (elingenawo ama-anticoagulant ku-Vacutainer®) ngokubhoboza imithambo.Vumela igazi ukuthi lihlule.Hlukanisa i-serum nge-centrifugation.Khipha ngokucophelela i-serum uyifake eshubhuni elisha elilebulwe ngaphambili.
Hlola amasampula ngokushesha ngangokunokwenzeka ngemva kokuqoqa.Gcina izibonelo ku-2°C-8°C uma zingahloliwe ngokushesha.
Gcina izibonelo ku-2°C-8°C kufika ezinsukwini ezi-5.Ama-specimens kufanele aqandiswe ku -20°C ukuze agcine isikhathi eside.
Gwema imijikelezo eminingi yokuqandisa.Ngaphambi kokuhlola, letha izibonelo eziqandisiwe ekamelweni lokushisa kancane futhi uhlanganise kahle.Izibonelo eziqukethe i-particle ebonakalayo kufanele zicaciswe nge-centrifugation ngaphambi kokuhlolwa.Ungasebenzisi amasampuli abonisa i-gross lipemia, i-gross hemolysis noma i-turbidity ukuze ugweme ukuphazamiseka ekuchazeni umphumela.
Letha isifanekiso nezingxenye zokuhlola ekamelweni lokushisa Xuba isifanekiso kahle ngaphambi kokuhlolwa uma sesincibilikile.Beka idivayisi yokuhlola endaweni ehlanzekile, eyisicaba.
Ngesampula yegazi eliphelele le-capillary:
Ukusebenzisa ithubhu le-capillary: Gcwalisa ishubhu le-capillary futhidlulisa cishe u-50µL (noma amaconsi angu-2) wegazi eliphelele lomunweisifanekiso kumthombo wesampula (S) wedivayisi yokuhlola, bese wengeza1 iconsi (cishe 30 µL)kweI-Diluent yesampulangokushesha emthonjeni wesampula.
Ngesampula yegazi lonke:
Gcwalisa i-dropper nge-specimen kedlulisa amaconsi ama-2 (cishe ama-50 µL)yesampula emthonjeni wesampula.Ukuqinisekisa ukuthi awekho amabhamuza omoya.Khona-kedlulisa iconsi elingu-1 (cishe 30 µL)Yesampula Ye-Diluent ngokushesha emthonjeni wesampula.
Okwesampula ye-Plasma/Serum:
Gcwalisa i-dropper nge-specimen kedlulisa iconsi elingu-1 (cishe 25 µL)yesampula emthonjeni wesampula.Ukuqinisekisa ukuthi awekho amabhamuza omoya.Khona-kedlulisa iconsi elingu-1 (cishe 30 µL) Yesampula Ye-Diluent ngokushesha emthonjeni wesampula.
Setha isibali sikhathi.Funda umphumela ngemizuzu eyi-15.Ungafundi umphumela ngemuva20 imizuzu.Ukuze ugweme ukudideka, lahla idivayisi yokuhlola ngemva kokuhumusha umphumela
| UMPHUMELA OMHLE:
| Ibhande elilodwa kuphela elinombala elivela endaweni yokulawula (C).Alikho ibhande elinemibala elibonakalayo elivela endaweni yokuhlola (T). |
| UMPHUMELA EMIBI:
| Amabhande amabili anombala avela kulwelwesi.Ibhendi eyodwa ivela endaweni yokulawula (C) kanti elinye ibhendi livela endaweni yokuhlola (T). |
| *QAPHELA: Ukushuba kombala endaweni yomugqa wokuhlola kuzohluka kuye ngokuhlangana kwamasosha omzimba angathathi hlangothi ku-COVID-19 kusifanekiso.Ngakho-ke, noma imuphi umthunzi wombala endaweni yomugqa wokuhlola kufanele ubhekwe njengebi. | |
|
UMPHUMELA ONGAvumelekile:
| Ibhendi yokulawula iyehluleka ukuvela.Imiphumela yanoma yikuphi ukuhlolwa okungakakhiqizi ibhande lokulawula ngesikhathi esishiwo sokufunda kufanele inganakwa.Sicela ubuyekeze inqubo bese uphinda ngokuhlolwa okusha.Uma inkinga iqhubeka, yeka ukusebenzisa ikhithi ngokushesha futhi uxhumane nomsabalalisi wangakini. |
1. Ukulawula Kwangaphakathi:Lokhu kuhlola kuqukethe isici sokulawula esakhelwe ngaphakathi, ibhendi ye-C.Ulayini u-C uthuthuka ngemva kokwengeza isifanekiso kanye ne-diluent yesampula.Uma kungenjalo, buyekeza yonke inqubo bese uphinda ukuhlola ngedivayisi entsha.
2. Ukulawula Kwangaphandle:I-Good Laboratory Practice incoma ukusebenzisa izilawuli zangaphandle, ezinhle nezingezinhle (ezihlinzekwe ngesicelo), ukuze kuqinisekiswe ukusebenza kahle kokuhlolwa.
